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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Yeast. 2006 Mar;23(4):279–291. doi: 10.1002/yea.1352

Figure 1.

Figure 1

Mapping of CRD1 gene transcription initiation site and the CRD1 promoter sequence. (A) DNA fragment containing the end of the 5′-untranslated region of the CRD1 gene. Lane 1, Promega 100 bp step marker. Lane 2, sample mRNA treated with RNase prior to the RACE reaction. Lane 3, 5′ RACE product from CRD1 mRNA. (B) Diagram of CRD1 promoter. EcoRI sites (single underline) used for the construction of gene fusions, transcription initiation start sites (arrow with +I), a potential UASINO (arrow), TATA box of putative promoter (overline) and the putative translation initiation codon (double underline)