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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Yeast. 2006 Mar;23(4):279–291. doi: 10.1002/yea.1352

Figure 4.

Figure 4

PCRD1 –lacZ expression in response to mitochondrial function. The parental strain DL1, a rho− mutant and pgs1A mutant (YCD4) all bearing plasmid pSD90 (PCRD1 –lacZ) were grown to mid-log phase in an inositol-free medium containing 2% glucose. Then half of each culture was maintained in glucose medium while the other half was washed and shifted to 3% glycerol/ethanol medium. Cells were harvested after 6 h and β-galactosidase activity was determined in whole cell extracts. Each value represents the mean of three experiments