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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Yeast. 2006 Mar;23(4):279–291. doi: 10.1002/yea.1352

Figure 5.

Figure 5

PCRD1–lacZ expression in crd1Δ and cds1Δ mutant strains. The parental strain (YPH98) and crd1Δ mutant (YCD2) with plasmid pSD90 (Pcrd1–lacZ) were grown to mid-log in a minimal medium with 2% galactose. β-galactosidase activity of the mutant was compared with that of the wild-type. The cds1Δ mutant YSD90A carrying a human cDNA encoding a CDP-DAG synthase (plasmid phCDS) under the control of PGAL1 promoter was transformed with plasmid pSD90 and cells were grown to mid-log phase in minimal medium with 2% galactose (to induce the plasmid-borne copy of the hCDS1 gene). β-galactosidase activity was assayed in the whole cell extracts and compared with that of the parental wild-type strain YPH102 (CDS) grown in the same medium. Each value represents the mean of three experiments