Table 1.
Yeast strains and plasmids
| Strains or plasmids | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| DL1 | MATa his3-11, 15 leu2-3, 12 ura3-251, 328, 372 | Van Loon et al. (1983) |
| rho− | MATa rho−, derivative of DL1 | This work |
| YCD4 | MATa pgs1::HIS3, derivative of DL1 | Chang et al. (1998a) |
| YPH98 | MATa ura3-52 lys2-801 ade2-101 leu2Δ1 trp1Δ1 | Sikorski and Hieter (1989) |
| YCD2 | MATa crd1::TRP1, derivative of YPH98 | Chang et al. (1998b) |
| YPH102 | MATα ura3-52 lys2-801 ade2-101 leu2Δ1 his3Δ200 | Sikorski and Hieter (1989) |
| YSD90A | MATα cds1::TRP1, derivative of YPH102 | Shen et al. (1996) |
| SH303 | MATa ino2::TRP1 his3 ura3 leu2 | S. A. Henry |
| SH307 | MATα ino4::LEU2 trp1 his3 ura3 | S. A. Henry |
| SH486 | his3 leu2 trp1 ura3 ino2Δ::TRP1 ino4::LEU2 | S. A. Henry |
| Plasmids | ||
| pMA109 | lacZ URA3 2μ and ColE1 origin | Anderson and Lopes (1996) |
| phCDS1 | PGAL1 –hCDS1 LEU2, 2μ and ColE1 origin | Shen and Dowhan (1997) |
| pSD80 | PDRC1–lacZ, derivative from pMA109 (inverted promoter) | This work |
| pSD90 | PCRD1–lacZ, derivative from pMA109 | This work |
| pSD91 | Derivative of pSD90, a 136 bp sequence including the putative UASINO was deleted from CRD1 promoter | This work |
| pSD92 | Derivative of pSD90, a 126 bp sequence up to the putative UASINO was deleted from CRD1 promoter | This work |
| pSD93 | Derivative of pSD92, a further 107 bp sequence up to the potential TATA box was deleted from CRD1 promoter | This work |