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. 2014 Jul 22;5:4413. doi: 10.1038/ncomms5413

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(a) Synthetic antagonist peptide Arg286, identified with protein painting, inhibited SAPK/JNK signalling downstream from IL1RI as effectively as IL1RAcP recombinant protein. In lane 8, scrambled peptide obtained by randomly shuffling Arg286 sequence does not inhibit the signalling downstream from IL1RI. Data are representative of three independent experiments. (b) Arg286 peptide inhibition of ligand pull-down within the receptor complex by His-tagged IL1RAcP. Schematic representation of the complex is depicted in insert. IL1RAcP in the absence of IL1RI does not pull down IL1β, lane 8. (c) Arg286 peptide mAb specific for IL1RAcP peptide extinguishes complex formation depicted in insert.