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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Biomed Microdevices. 2010 Oct;12(5):855–863. doi: 10.1007/s10544-010-9440-3

Fig. 4.

Fig. 4

Phenotypic characteristics of aAPC and propagation of human T cells on aAPC. (a) Surface expression of co-stimulatory molecules and adsorbed OKT3 on K562-aAPC. Open histograms show the expression on K562 parental cells and filled histograms show the expression on genetically modified K562 which function as aAPC. Expression of CD64, CD86, CD137L, membrane-bound IL-15, and OKT3 were detected with antibodies. (b) Propagation of primary T cells on aAPC. Irradiated OKT3-loaded K562-aAPC were added to the T-cell culture for every 7 days at two different ratios. (c) T-cell subsets after 7 and 14 days of numeric expansion on OKT3-loaded aAPC at 1 : 1 (T cells : aAPC) ratio