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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Nature. 2013 Feb 17;494(7438):497–501. doi: 10.1038/nature11884

Figure 1. Mediator confers the ncRNA-as dependent activation of a heterologous reporter.

Figure 1

a, Schematic representation of genomic control insert (top) or ncRNA-a7 insert (bottom) in luciferase reporter vector driven by a TK-promoter.

b, Depletion of ncRNA-a7 reduces the luciferase activity in the ncRNA-a7 luciferase reporter cell lines.

c, Depletion of the transcription factors or enhancers in the control or ncRNA-a7 reporter cell lines. The red bar signifies reduced transcription using siRNA against MED12, comparable with siRNA against ncRNA-a7.

d, Depletion of different Mediator subunits using the ncRNA-a7 reporter cell lines.

e, Depletion of the Mediator subunits in ncRNA-a1 or ncRNA-a3 luciferase reporter cell lines. All data shown are mean ±SEM of three independent experiments. **p<0.01, ***p<0.001 by two-tailed Student’s T-test.