Figure 1.

Podocyte growth under nonpermissive (37°C) condition. At 50–60% confluence, nondifferentiated cells were placed from 33°C to 37°C to induce differentiation which was followed by video microscopy for 14 days. Cell numbers were determined by using time-lapse phase contrast images that were acquired one frame every 10 minutes. Graph is a representative of 3 experiments with similar results.