TABLE II. Frequency of the V1016I knockdown resistance mutation in the populations of Madeira, Brazil and Venezuela analysed in this study.

Population	Mosquitoes analysed (n)	V/V a (n)	V/I b (n)	I/I c (n)	I d (%)
Madeira	69	59	9	1	0.08
Brazil	32	27	4	1	0.09
Venezuela	30	16	14	0	0.23

a : number of individuals homozygous for the susceptible-associated allele; b : number of heterozygous individuals; c : number of individuals homozygous for the resistant-associated allele; d : allele frequency for the resistant-associated allele. Of the total 131 individuals genotyped, 35 were confirmed by sequencing. I: isoleucine; V: valine.