FIGURE 2. Impaired adipogenesis in C/EBPβ^−/− x Lepr^db/db mice.

A–D, portions of gonadal adipose tissue were removed from anesthetized mice, fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned at 4 µM thickness. Sections were stained with hematoxylin and eosin and photographed at 50× magnification. The bar in the lower right corner of each photo equals 100 µm.E, for immunohistochemistry, sections were deparafinized in xylenes and rehydrated, and antigen retrieval was performed in heated citrate buffer. Sections were immunoblotted with Pref-1 antibody.