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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: J Biol Chem. 2007 Mar 26;282(21):15717–15729. doi: 10.1074/jbc.M701329200

FIGURE 7. Dominant negative C/EBPβ (LIP) and C/EBPβ shRNA prevent TG accumulation in FAO cells.

FIGURE 7

FAO cells were transduced 24 h after plating with Adv-GFP or Adv-LIP (30 pfu/cell) and treated with or without 200 µM palmitate for 24–48 h before harvesting.A, cellular TG levels. *, p < 0.05 versus control. B, relative mRNA levels for PPARγ, PPARα, and C/EBPβ quantified by real-time quantitative PCR. RNA expression data were normalized to the levels of 18S RNA and expressed as percent change over control. Data represent the mean ± S.E. of two independent experiments performed in triplicate. *, p < 0.05 versus control. C, immunoblot showing knockdown of C/EBPβ LAP and LIP isoforms with Adv-C/EBPβ-shRNA. FAO cells were treated with adenovirus-delivered shRNA targeting C/EBPβ (Adv-C/EBPβ-shRNA) for 24 h and treated with or without 200 µM palmitate for 48 h before harvesting. D, cellular TG levels. *, p < 0.05 versus control.