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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: J Virol Methods. 2011 Mar 17;174(0):29–34. doi: 10.1016/j.jviromet.2011.02.025

Figure 1.

Figure 1

Detection of YFV sequences in extracts of infected Vero cells. (a) Size fractionated, ethidium bromide labeled product (4μL) of RT-(lanes 1 to 7) and RT-hemi- nested-PCR (lanes 8 to 14) steps (1% agarose gel). (b) the dissociation curve for the SYBR® qRT-PCR showing the melting temperature for positive YFV genome detection ranging from 79°C to 81°C (mean of 80°C). “C+1 and C+2” stand for positive controls used during the RT-PCR and Hemi-nested-PCR steps, respectively. “NC” stands for negative control (RNA extracted from supernatant of uninfected Vero cells) and “MW” stands for molecular weight marker (100bp DNA ladder, Invitrogen, Carlsbad, CA, USA).