Detection of YFV sequences by RT-PCR and RT-hemi-nested-PCR and SYBR® qRT-PCR in serum from an infected hamster (panels a and b) and limits of detection using RT-PCR, RT-hemi-nested-PCR and SYBR® qRT-PCR methods (c). “+” stands for a positive result, “-“ for a negative result, “U” for undiluted sample, “NC” for negative control (RNA extracted from VERO uninfected cells), and “MW” for molecular weight marker (Low DNA mass ladder, Invitrogen, Carlsbad, CA, USA).