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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: J Virol Methods. 2011 Mar 17;174(0):29–34. doi: 10.1016/j.jviromet.2011.02.025

Table 3.

Performance of RT-PCR, RT-hemi-nested-PCR, qRT-PCR, classical RT-PCR and virus isolation in detection of YFV in infected experimentally hamsters.

Standard protocol Current protocol New protocols
 Serology
RT-Hemi-Nested-PCR (a/b)
Days post infection Virus isolation RT-PCR (a/b) RT-PCR step Nested-PCR step qRT-PCR (a/b) MAC-ELISA positive(a/b)
Day 0 25/25 25/25, S=100% 25/25, S=100% 25/25, S=100% 25/25, S=100% 0/25
Day 1 25/25 25/25, S=100% 25/25, S=100% 25/25, S=100% 25/25, S=100% 0/25
Day 2 25/25 25/25, S=100% 25/25, S=100% 25/25, S=100% 25/25, S=100% 0/25
Day 3 25/25 20/25, S=80% 21/25, S=84% 25/25, S=100% 25/25, S=100% 0/25
Day 4 25/25 18/25, S=72% 21/25, S=84% 24/25, S=96% 25/25, S=100% 1/25
Day 5 23/25 13/25, S=52% 17/25, S=68% 21/25, S=88.8% 22/25, S=96% 3/25
Day 6 10/25 2/25, S=8% 5/25, S=20% 12/25, S=48% 11/25, S=44% 10/25
Day 7 1/25 0/25, S=0% 0/25, S=0% 2/25, S=8% 4/25, S=16% 25/25
a

:positive

b

:tested

High sensitivity

graphic file with name nihms-587661-t0004.jpg Medium sensitivity

graphic file with name nihms-587661-t0005.jpg Low sensitivity

graphic file with name nihms-587661-t0006.jpg Very low sensitivity