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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Nat Med. 2008 Sep;14(9):931–938. doi: 10.1038/nm.1857

Figure 1.

Figure 1

Synaptopodin specifically interacts with 14-3-3. (a) Schematic of synaptopodin isoforms. The white box shows the first 676 amino acids that are shared between Synpo-long and Synpo-short. This fragment contains two 14-3-3 binding motifs (black ovals) and two CatL cleavage sites (arrows). (b) In the adult mouse kidney, 14-3-3β colocalizes with synaptopodin in podocytes. Scale bar, 30 μm. (c) In differentiated cultured podocytes, 14-3-3β colocalizes with synaptopodin along stress fibers (top), and both proteins remain associated after disruption of actin filaments with latrunculin A (bottom). Scale, 25 μm. (d) Synaptopodin from isolated mouse glomerular extracts (input) specifically binds GST–14-3-3β but not GST alone. (e) Coimmunoprecipitation experiments show that endogenous synaptopodin interacts with endogenous 14-3-3β in isolated mouse glomeruli. An antibody to GFP serves as negative control. IP, immunoprecipitation. (f) GFP–14-3-3β precipitates with wild-type (WT) Flag-synaptopodin and phosphomimetic Flag–Synpo-ED but not with phosphoresistant Flag–Synpo-AA or Flag-raver (control).