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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Nat Med. 2008 Sep;14(9):931–938. doi: 10.1038/nm.1857

Figure 2.

Figure 2

Identification of synaptopodin as calcineurin binding protein. (a) GFP–Synpo-short and GFP–Synpo-alt precipitate with Flag-tagged aCnA from co-transfected HEK293 cells. GFP–Synpo-alt can also interact with WT calcineurin. No binding is found with Flag-raver (control). (b) Endogenous coimmunoprecipitation experiments show that synaptopodin interacts with calcineurin in isolated mouse glomeruli. An antibody specific for GFP serves as negative control. (c) In the adult mouse kidney (top) and differentiated cultured podocytes (bottom), calcineurin partially colocalizes with synaptopodin. Scale bars, 30 μm (top panels) and 25 μm (bottom panels).