Figure 7. Phosphorylation status of GSK-3 substrates.
(A–A') Western blots of P0 cortical lysates from Gsk3loxp:Neurod6 mutants and wild-type controls performed in triplicate. Levels of GSK-3 proteins and phospho-target proteins are shown. Strong reductions in phosphorylation of doublecortin on ser327/Thr321 and CRMP-2 on Thr514 are evident. (A') Quantification of relative densities from A. p values shown in figure (n = 3, unpaired t-test) (B–B') Western blots of cortical lysates at P0 showing levels of other GSK-3 targets. No changes were observed in phosphorylation of dynamin, pCREB, or pFAK. No change was observed in cleaved caspase-3. GAPDH was used as a loading control. (B') Quantification of relative protein densities (n = 3, unpaired t-test).