Table 1.
Genes of the genome island and assigned functions in the metabolism of monoterpenes in Castellaniella defragrans 65Phen
| Protein_id | Proteine detection method a | 2D fold change/LC enrichment level b | Gene annotation |
Related gene product |
|||
|---|---|---|---|---|---|---|---|
| E value | % identity | Organism | Accession no. | ||||
|
CDM25240 |
n.d. |
n.d. |
Hypothetical protein |
4E-60 |
59 |
Thauera terpenica 58Eu |
EPZ16239 |
|
CDM25241 |
LC |
++ |
Acyl-CoA dehydrogenase protein |
0.0 |
82 |
Thauera terpenica 58Eu |
EPZ16240 |
|
CDM25242 |
LC |
0 |
Molybdopterin-binding OR |
3E-70 |
74 |
Thauera terpenica 58Eu |
EPZ16227 |
|
CDM25243 |
n.d. |
n.d. |
Tyrosine/serine phosphatase |
1E-58 |
48 |
Thauera linaloolentis 47Lol |
ENO83508 |
|
CDM25244 |
LC |
0 |
2,4-dienoyl-CoA reductase |
0.0 |
87 |
Thauera terpenica 58Eu |
EPZ16243 |
|
CDM25245 |
n.d. |
n.d. |
NADH:flavin oxidoreductase |
1E-179 |
68 |
Thauera terpenica 58Eu |
EPZ16244 |
|
CDM25246 |
2D/LC |
3.0/++ |
3-hydroxyacyl-CoA dehydrogenase |
5E-167 |
82 |
Azoarcus sp. KH32C |
YP_007598290 |
|
CDM25247 |
n.d. |
n.d. |
IS4 family transposase |
0.0 |
63 |
Thiomonas sp. FB-6 |
WP_018915433 |
|
CDM25248, MrcH |
n.d. |
n.d. |
MaoC-like dehydratase |
2E-36 |
62 |
Azoarcus sp. KH32C |
YP_007598291 |
|
CDM25249, MrcG |
n.d. |
n.d. |
MaoC-like dehydratase |
6E-50 |
67 |
Thauera terpenica 58Eu |
EPZ16257 |
|
CDM25250, MrcF |
LC |
++ |
Perillyl-CoA hydratase |
0.0 |
58 |
Thauera terpenica 58Eu |
EPZ16258 |
|
CDM25251, MrcE |
2D/LC |
18/++ |
4-isopropenyl-2-oxo-cyclohexane-1-carboxyl-CoA hydrolase |
4E-167 |
88 |
Azoarcus sp. KH32C |
YP_007598294 |
|
CDM25252, MrcD |
2D/LC |
3.0/++ |
2-hydroxy-4-isopropenyl-cyclohexane-1-carboxyl-CoA dehydrogenase |
1E-153 |
85 |
Azoarcus sp. KH32C |
YP_007598295 |
|
CDM25253, MrcC |
2D |
2,3 |
2,4-dienoyl reductase |
0.0 |
86 |
Thauera terpenica 58Eu |
EPZ16261 |
|
CDM25254, MrcB |
LC |
++ |
Acyl-CoA dehydrogenase |
0.0 |
90 |
Azoarcus toluclasticus |
WP_018990727 |
|
CDM25255, MrcA |
LC |
++ |
Oxidoreductase, FAD-binding |
0.0 |
73 |
Azoarcus toluclasticus |
WP_018990723 |
|
CDM25256 |
2D/LC |
19/++ |
(R)-specific enoyl-CoA hydratase |
1E-88 |
85 |
Thauera terpenica 58Eu |
EPZ15051 |
|
CDM25257 |
LC |
0 |
Citrate lyase |
6E-141 |
73 |
Thauera terpenica 58Eu |
EPZ15052 |
|
CDM25258 |
LC |
++ |
Acyl-CoA dehydrogenase |
0.0 |
92 |
Thauera terpenica 58Eu |
EPZ15053 |
|
CDM25259 |
LC |
++ |
RND efflux transporter |
0.0 |
62 |
Thauera terpenica 58Eu |
EPZ15054 |
|
CDM25260 |
LC |
++ |
RND efflux transporter |
0.0 |
80 |
Thauera terpenica 58Eu |
EPZ15055 |
|
CDM25261 |
LC |
++ |
RND efflux transporter |
4E-142 |
68 |
Thauera terpenica 58Eu |
EPZ15056 |
|
CDM25262 |
LC |
++ |
RND efflux transporter |
0.0 |
81 |
Thauera terpenica 58Eu |
EPZ15057 |
|
CDM25263 |
LC |
++ |
Acetoacetyl-CoA synthetase |
0.0 |
84 |
Thauera terpenica 58Eu |
EPZ15058 |
|
CDM25264 |
n.d. |
n.d. |
Enoyl-CoA hydratase |
9E-136 |
76 |
Thauera terpenica 58Eu |
EPZ15059 |
|
CDM25265, GeoC |
LC |
++ |
Perillate--CoA ligase |
0.0 |
71 |
Thauera terpenica 58Eu |
EPZ15060 |
|
CDM25266 |
LC |
0 |
Hypothetical protein |
4,3 |
52 |
Fusarium graminearum PH-1 |
XP_382023 |
|
CDM25267, GeoA |
2D/LC |
42/++ |
Geraniol dehydrogenase |
0.0 |
84 |
Thauera terpenica 58Eu |
EPZ14350 |
|
CDM25268 |
LC |
++ |
Hypothetical protein |
8E-117 |
74 |
Thauera terpenica 58Eu |
EPZ14349 |
|
CDM25269 |
n.d. |
n.d. |
Hypothetical protein |
2E-97 |
69 |
Thauera terpenica 58Eu |
EPZ14348 |
|
CDM25270 |
n.d. |
n.d. |
Hypothetical protein |
6E-32 |
66 |
Thauera terpenica 58Eu |
EPZ14347 |
|
CDM25271 |
n.d. |
n.d. |
Thioesterase |
2E-33 |
46 |
Magnetospirillum magneticum |
YP_420191 |
|
CDM25272, LDI |
LC |
++ |
LDI precursor protein |
8E-14 |
25 |
Stereum hirsutum FP-91666 SS1 |
EIM80109 |
|
CDM25273 |
n.d. |
n.d. |
Hypothetical protein |
5E-85 |
43 |
Gordonia paraffinivorans |
WP_006900876 |
|
CDM25274 |
n.d. |
n.d. |
Hypothetical protein |
5E-07 |
30 |
Gordonia paraffinivorans |
WP_006900845 |
|
CDM25275 |
LC |
++ |
Acyl-CoA dehydrogenase |
4E-106 |
54 |
Azoarcus toluclasticus |
WP_018990670 |
|
CDM25276 |
n.d. |
n.d. |
Hypothetical protein |
4E-40 |
40 |
Glaciecola punicea |
WP_006005307 |
|
CDM25277 |
n.d. |
n.d. |
Hypothetical protein |
5E-21 |
58 |
Pseudomonas sp. 19-rlim |
AEO27370 |
|
CDM25278 |
n.d. |
n.d. |
Hypothetical protein |
1E-69 |
62 |
Pseudomonas sp. 19-rlim |
AEO27371 |
|
CDM25279 |
LC |
++ |
Hypothetical protein |
1E-120 |
68 |
Pseudomonas sp. 19-rlim |
AEO27372 |
|
CDM25280 |
n.d. |
n.d. |
MarR transcriptional regulator |
7E-81 |
83 |
Thauera terpenica 58Eu |
EPZ16291 |
|
CDM25281, GeoB |
2D/LC |
15/++ |
Geranial dehydrogenase |
0.0 |
91 |
Thauera terpenica 58Eu |
EPZ16290 |
|
CDM25282 |
LC |
++ |
Acyl-CoA dehydrogenase |
0.0 |
89 |
Thauera terpenica 58Eu |
EPZ16289 |
|
CDM25283 |
n.d. |
n.d. |
LuxR family transcriptional regulator |
0.0 |
59 |
Thauera terpenica 58Eu |
EPZ16271 |
|
CDM25284, CtmG |
LC |
++ |
Hypothetical protein |
5E-35 |
39 |
Azoarcus sp. KH32C |
YP_007598506 |
|
CDM25285, CtmF |
LC |
++ |
NADH:ferredoxin oxidoreductase |
4E-147 |
56 |
Caulobacter sp. AP07 |
WP_007674692 |
|
CDM25286, CtmE |
2D |
6.3/++ |
Ferredoxin, 2Fe-2S |
1E-32 |
50 |
Caulobacter crescentus CB15 |
NP_422318 |
|
CDM25287, CtmD |
n.d. |
n.d. |
Hypothetical protein |
4,1 |
38 |
Ochrobactrum sp. CDB2 |
WP_007881652 |
|
CDM25288, CtmC |
n.d. |
n.d. |
Hypothetical protein |
2,3 |
33 |
Bombus impatiens |
XP_003489707 |
|
CDM25289, CtmB |
2D/LC |
3.4/++ |
Limonene dehydrogenase |
4E-131 |
41 |
Deltaproteobacterium NaphS2 |
WP_006422074 |
|
CDM25290, CtmA |
LC |
++ |
Limonene dehydrogenase |
5E-57 |
30 |
Deltaproteobacterium NaphS2 |
WP_006422074 |
|
CDM25291 |
LC |
++ |
Acetyl-CoA acetyltransferase |
0.0 |
79 |
Thauera terpenica 58Eu |
EPZ16237 |
|
CDM25292 |
LC |
0 |
MarR transcriptional regulator |
2E-76 |
77 |
Thauera terpenica 58Eu |
EPZ16235 |
|
CDM25293 |
n.d. |
n.d. |
Hypothetical protein |
4E-24 |
56 |
Thauera terpenica 58Eu |
EPZ16283 |
|
CDM25294 |
LC |
++ |
Hypothetical protein |
2E-83 |
68 |
Thauera terpenica 58Eu |
EPZ16282 |
|
CDM25295 |
n.d. |
n.d. |
Hypothetical protein |
6E-155 |
76 |
Thauera terpenica 58Eu |
EPZ16281 |
|
CDM25296 |
n.d. |
n.d. |
MarR transcriptional regulator |
5E-94 |
81 |
Thauera terpenica 58Eu |
EPZ16280 |
|
CDM25297 |
n.d. |
n.d. |
Hypothetical protein |
2E-168 |
72 |
Thauera terpenica 58Eu |
EPZ16232 |
|
CDM25298 |
LC |
++ |
Hypothetical protein |
0.0 |
72 |
Thauera terpenica 58Eu |
EPZ16231 |
|
CDM25299 |
LC |
++ |
Acetyl-CoA acetyltransferase |
0.0 |
90 |
Thauera terpenica 58Eu |
EPZ16230 |
|
CDM25300 |
n.d. |
n.d. |
Acetyl-CoA hydrolase/transferase |
0.0 |
74 |
Thauera terpenica 58Eu |
EPZ16229 |
|
CDM25301 |
LC |
++ |
Electron transfer flavoprotein |
7E-133 |
78 |
Thauera terpenica 58Eu |
EPZ16226 |
| CDM25302 | n.d. | n.d. | Electron transfer flavoprotein | 7E-160 | 76 | Azoarcus sp. KH32C | YP_007552025 |
a2D, identified with 2D-SDS-PAGE and MALDI-TOF-MS; LC, identified with LC-ESI-MS/MS.
b++ peptides only identified in α-phellandrene fraction, + increase in α-phellandrene fraction, 0 ratio remained unchanged.