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Colocalization analysis of Cav-3 WT and Cav-3 V82I with the ER marker calnexin. BHK cells were transiently transfected with Cav-3 WT (top) or Cav-3 V82I (bottom) and subjected to double labelling with antibodies raised against caveolin-3 (green) and calnexin (red). As shown in the merged images, no significant colocalization with calnexin was observed for both wilt-type and mutated caveolin-3 proteins. Data are representative of three independent cultures.
