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. 2014 Jul 21;12:42. doi: 10.1186/1477-5956-12-42

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Copyright © 2014 Zhao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Workflow of protein identification in the FSGS rat model. Urine was collected at days 0, 3, 7, 11, 15 and 23 after ADR administration, and urinary proteins were enriched by ConA agarose followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) identification. Several proteins were verified in ten more rats by western blot analysis.