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. 2014 Jul 23;55(7):4568–4576. doi: 10.1167/iovs.13-13127

Figure 2.

Figure 2

Lack of cytoprotective effect of FA in PPARα−/− OIR mice. (A) Retinal GFAP expression in P17 RA and OIR mice treated with Veh or FA was measured by Western blot analysis, semiquantified by densitometry, normalized by β-actin levels, and expressed as the ratio to that in the RA control (n = 6). (B) Deoxyribonucleic acid fragmentation in mouse retains treated as above was measured by cell death ELISA in retinal homogenates at P17 (n ≥ 5). (C) Apoptotic cells in retinal sections from mice treated as above were labeled by TUNEL (red), and the nuclei counterstained with DAPI (blue). TUNEL-positive retinal cells were counted in retinal sections (n ≥ 6); scale bars: 10 μm. (D) Retinal sections were double stained with the anti-CD31 antibody (green) and TUNEL (red), and the nuclei were counterstained with DAPI (blue; n ≥ 3). Scale bars: 10 μm. All values are mean ± SEM. *P ≤ 0.05, ****P ≤ 0.0001.