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Protection of R28 cells by FA against hypoxia in vitro. (A) R28 cells were treated with CoCl2, Veh, or FA for 48 hours. Apoptotic cells were stained with TUNEL (red), and the nuclei counterstained with DAPI (blue). TUNEL-positive cells in each slide chamber were counted and expressed as percentage of total cells (mean ± SEM; n = 3). ***P ≤ 0.001.
