Figure 3.

Evidence of microclot formation, neuronal apoptosis, and CPP depletion after experimental SAH. Quantification of TUNEL-positive cells and fibrinogen-positive stained microthrombi in cortex and hippocampus. (a) There were significant increases in the number of TUNEL-positive cells in both cerebral cortex and hippocampus as compared to controls (P = 0.016 and P = 0.017, resp., Student's t-test). (b) There was a nonstatistically significant trend towards an increase in fibrinogen-positive microvessels in the cerebral cortex (P = 0.06) and a statistically significant increase in fibrinogen-positive microvessels in the hippocampus (P = 0.03). No correlations of TUNEL-positivity with fibrinogen-positivity were observed in cortex (R ² = 0.094; (c)) or in hippocampus (R ² = 0.2; (d)). All data are expressed as mean ± SEM, n = 8 in SAH group, and n = 4 in control group. A P value of <0.05 was considered statistically significant. (e) Time course of the CPP depletion after induction of SAH compared to control animals.