Fig. 3.
Knockdown of 5-HT1F receptor protein in RPTC by siRNA transfection at 72 hours (A) Representative immunoblot and quantification of 5-HT1F receptor protein knockdown. (B) Mitochondrial protein levels after 5-HT1F receptor knockdown. (C) Decreased 5-HT1F receptor levels block LY334370- and LY344864-induced MB. RPTC were treated with scramble or siRNA for 72 hours and treated with LY334370 or LY344864 for 24 hours. Data are X + S.E.M., n ≥ 3. *Significantly different from scrambled or 5-HT1F receptor agonist treatment (P < 0.05). GAPDH, glyceraldehyde 3-phosphate dehydrogenase.