Fig. 2.
Effect of PTZ and DTG on ICa is mediated by σ1R. After whole-cell configuration, the ICa was measured with a square wave voltage command (holding potential at −100 mV and step to 0 mV for 50 milliseconds). Cells were preincubated with BD1047 or BD1063 for 15 minutes before PTZ and DTG administration. (A) Sample traces from control animal showed that DTG (10 μM) inhibited ICa and the inhibition was prevented by BD1063 (10 μM). (B) Sample traces from control animal showed that PTZ (100 μM) inhibited ICa and the inhibition was prevented by BD1047 (10 μM). Summary data of ICa inhibition were derived from the current density, which was normalized to its baseline current density. Mean ± S.E.M.; number in bars represents the sample size; ***P < 0.001. BL, baseline.