Fig. 3.

Rimonabant precipitated cannabinoid withdrawal responses following prolonged MAGL inhibition, regardless of FAAH blockade. (A) Rimonabant precipitated paw-fluttering behavior in mice injected repeatedly with high-dose JZL184 (40 mg/kg), with or without repeated treatment with high doses of the FAAH inhibitor PF-3845 (10 mg/kg) or the dual inhibitor JZL195 (20 mg/kg). (B) Rimonabant precipitated head shakes in mice treated repeatedly with JZL195 only. (C and D) Repeated JZL184 but not repeated vehicle produced similar precipitated paw-fluttering response in FAAH(+/+) and (−/−) mice, but did not significantly affect head-shaking behavior in either genotype. **P < 0.01; ***P < 0.001 versus vehicle controls; n = 8–12 per group.