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. 1995 Sep 12;92(19):8999–9003. doi: 10.1073/pnas.92.19.8999

Targeting cancer micrometastases with monoclonal antibodies: a binding-site barrier.

T Saga 1, R D Neumann 1, T Heya 1, J Sato 1, S Kinuya 1, N Le 1, C H Paik 1, J N Weinstein 1
PMCID: PMC41095  PMID: 7568060

Abstract

Monoclonal antibodies penetrate bulky tumors poorly after intravenous administration, in part because of specific binding to the target antigen. Experiments presented here demonstrate an analogous phenomenon in micrometastases; poor antibody penetration, attributable to a "binding-site barrier" phenomenon, can be seen in guinea pig micrometastases as small as 300 microns in diameter. Increasing the dose of antibody can partially overcome this limitation, but at a cost in specificity.

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