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. 2014 Jun 24;124(8):3391–3406. doi: 10.1172/JCI72517

Figure 6. Pair-feeding fails to fully recapitulate the effect of pNAPE-EcN treatment on body weight and body fat gain.

Figure 6

A group of mice treated with pEcN (E) were were pair-fed (PF) by restricting them to the same number of calories of food as the pNAPE-EcN mice (N). An ad libitum–fed control group received standard drinking water (W). All values are the mean ± SEM; n = 9–10 mice per group. (A) Body weight gain during 4 weeks of treatment and pair-feeding. Two-way RM ANOVA, P < 0.0001 for time, P = 0.0003 for treatment. P < 0.05 by Bonferroni’s multiple comparison test for pair-fed versus pNAPE-EcN for days 16 to 25. P < 0.05 for pair-fed versus pEcN and versus standard water for day 28. (B) Body fat at week 0 (before beginning treatment) and week 4 (28 days after beginning treatment). No group differences were observed at week 0. Week 4 1-way ANOVA, P < 0.0001 by 1-way ANOVA; P < 0.05 by Dunnett’s multiple comparison test for pair-fed versus water or versus pEcN. Pair-fed versus pNAPE-EcN, not significant. (C) Calculated feeding efficiency, P < 0.0001 by 1-way ANOVA; P < 0.05 BY Dunnett’s multiple comparison test for pair-fed versus pEcN; pair-fed versus standard water or versus pNAPE-EcN, not significant.