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. 2014 Jul 18;124(8):3311–3324. doi: 10.1172/JCI69625

Figure 4. Accumulation of I11-stained IAPP oligomer in primary monkey islet cells.

Figure 4

(A) Primary monkey islet cells or mouse islet cells were incubated with 100 nM bafilomycin (BAF) that efficiently blocks autophagy of primary cells or solvent alone (Con) for 24 hours. Confocal microscopy was performed after immunofluorescent staining using I11 Ab. Scale bar: 5 μm. (B) The number of I11-stained puncta was counted. (C) After the same incubation of primary monkey and mouse islet cells with or without bafilomycin, apoptosis was determined by measuring oligonucleosomal content in cell extract using a Cell Death Detection ELISA Kit (Roche). **P < 0.01, ***P < 0.001.