Figure 6. Innate sensing of allogeneic non-self precipitates graft rejection.

(A) Heart grafts from multiple donor strains (shown on x axis) were transplanted into B6 RAG^–/–γc^–/–CX₃CR1^gfp/+ recipients, and host-derived mature mono-DCs present in the grafts were enumerated by flow cytometry 3 days later. (B and C) Graft recipients, as in A, received 5 × 10⁵ OT-II cells 2 days after transplantation. Grafts and spleens were harvested on the day of rejection or at termination of the experiment (day 42). IFN-γ production by OT-II cells in the spleen is shown in B. Allograft survival, number of graft-infiltrating T cells, and representative graft sections stained with anti-CD3 (red) and hematoxylin (blue) are shown in C (original magnification, ×2). *P < 0.05 compared with B6 and B6-OVA groups.