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. 2014 Jul 18;124(8):3325–3338. doi: 10.1172/JCI73932

Figure 2. Deletion of Atm in endothelial cells does not affect tumor growth or vascular development of primary soft tissue sarcomas.

Figure 2

(A) Time for sarcomas to reach 200 mm3 after intramuscular injection of adeno-FlpO in KPFRTVAtmfl/+ and KPFRTVAtmfl/fl mice (n = 25 per group). (B) Tumor growth curves and (C) time to tripling for unirradiated sarcomas in KPFRTVAtmfl/+ and KPFRTVAtmfl/fl mice (n = 10 per group). (D and E) Immunofluorescence (D) and quantification (E) of endothelial cell marker CD31 in sarcomas from KPFRTVAtmfl/+ and KPFRTVAtmfl/fl mice (n = 10 per group). (F and G) Immunofluorescence (F) and quantification (G) of hypoxia marker EF5 in sarcomas from KPFRTVAtmfl/+ and KPFRTVAtmfl/fl mice (n = 10 per group). Regions of low and high hypoxia are shown for both genotypes. All data are mean ± SEM. Scale bars: 100 μm (D and F).