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. 2014 Jul 6;2014:564285. doi: 10.1155/2014/564285

Figure 4.

Figure 4

Analysis of IgG isotype profile in serum of mice immunized with the recombinant proteins. Sera from BALB/c mice immunized with the recombinant proteins alone or in fusion with DnaK were analyzed by ELISA. IgG, IgG1, and IgG2a titers were evaluated in each case. Sera from PBS injected mice were employed as negative control. Statistical analyses were performed by two-tailed t-test, comparing the titer obtained with leptospiral surface protein alone with the corresponding DnaK fusion protein.