Figure 5.

Analysis of lymphocyte proliferation in cultured splenocytes from mice immunized with recombinant proteins. Splenocytes from immunized mice were isolated and cultured for 48 h. The cells were stimulated with the recombinant protein alone or in fusion with DnaK. Cells were further incubated with BrdU and DNA synthesis was quantified by BrdU immunodetection kit (M&M). ConA and culture medium were employed as positive and negative stimulation controls, respectively (not shown). The data are from two independent experiments.