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. 2014 Jul 6;2014:564285. doi: 10.1155/2014/564285

Table 1.

Gene locus, protein given name, NCBI reference sequence number, features, sequence of the primers employed for DNA amplification, and molecular mass of expressed recombinant proteins.

Gene locus1 Recombinant protein given name NCBI reference sequence number2 Description/function Sequence of primers for PCR amplification and restriction cloning sites Recombinant protein molecular mass
LIC10524 DnaK YP_000508 Molecular chaperone DnaK F: 5′GGATCCGCTATGGAAGGTGGAGACCCGGTC3′ (BamHI) 
R: 5′CCATGG    GATATC  TCACAGCTG  CGGACCCGGACCCTTTTTCTCATCGTCTAC3′ 
(NcoI, EcoRV and PvuII)		 68.9 kDa
LIC10368 Lsa21a YP_000355 Putative lipoprotein F: 5′GGATCC    CAGCTG  GCTATGG  CCTGTCCGGATGAAAAAAAAG3′ 
(BamHI and PvuII) 
R: 5′CCATGG    GATATCTCAAAATACATTCACACGAATATCTC3′ 
(NcoI and EcoRV)		 19.8 kDa
LIC10494 rLIC10494b YP_000478 Putative lipoprotein F: 5′GGATCC    CAGCTG  GCTATGG  TAATCTTTGGCAACAAAAC3′ 
(BamHI and PvuII) 
R: 5′CCATGG    GATATC  TCA  TTTGTTTTCAGATTTTTTC3′ 
(NcoI and EcoRV)		 25.1 kDa
LIC12690 Lp95 c-terminalc YP_002611 Putative lipoprotein with a domain of unknown function (DUF1554) F: 5′GGATCC    CAGCTG  GCTATGG  AAAATGGAGAAGACGTTAC3′ 
(BamHI and PvuII) 
R: 5′CCATGG    GATATC  TCA  TTGTTCCACACAAAGAATG3′ 
(NcoI and EcoRV)		 43.6 kDa
LIC12730 rLIC12730d YP_002650 Hypothetical protein with TPR motif F: 5′GGATCC    CAGCTG  GCTATGG  GACTTCCCAATTTCTCTATTC3′  (BamHI and PvuII) 
R: 5′CCATGG    GATATC  TCA  TTTCGTAAAAAATCCAGCTTC3′  (NcoI and EcoRV)		 75.7 kDa

aAtzingen et al., 2008 [18], Atzingen et al., 2012 [28].

bAtzingen et al., 2010 [20], Vieira et al., 2010 [29].

cAtzingen et al., 2009 [19].

dAtzingen et al., 2010 [20], Vieira et al., 2010 [29], Felix et al., 2011 [30].