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. 2014 Jul 24;8:196. doi: 10.3389/fncel.2014.00196

Figure 1.

Figure 1

KCl-induced activity decreases GSK3 β phosphorylation of CRMP2 without changing GSK3 β activity or expression. (A) Top: Timeline of experimental procedures. Bottom: Schematic of GSK3β and Cdk5 phosphorylation sites within the rat CRMP2 sequence. Numbers represent amino acid residues within the CRMP2 sequence. Representative immunoblots of GSK3β-phosphorylated CRMP2 (CRMP2 pThr509/pThr514), total CRMP2, and β III-tubulin (B), inactivated Ser9-phosphorylated GSK3β and β III-tubulin (E), and total GSK3β and β III-tubulin (G) from naïve cortical neurons compared to those exposed to KCl for 30 min or 96 h. Summary of the relative levels of the indicated proteins (C,D,F,H). Expression of GSK3β-phosphorylated CRMP2 (CRMP2 pThr509/pThr514) is decreased following 30 min or 96 h exposure to KCl (C) whereas total CRMP2 expression was not affected by KCl exposures (D) (*p < 0.05 compared to control; One-Way ANOVA, Tukey's post-hoc analysis) (n = 4). KCl treatment did not alter phosphorylation of GSK3β (F) nor total GSK3β expression (H) did not change following 30 min or 96 h KCl treatment (One-Way ANOVA, Tukey's post-hoc analysis) (n = 5).