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. 2014 Jul 24;8:196. doi: 10.3389/fncel.2014.00196

Figure 5.

Figure 5

(S)-LCM subverts CRMP2-mediated tubulin polymerization. (A) Effects of enantiomers of LCM on CRMP2-mediated microtubule assembly were measured by light scattering and absorbance at 412 nm. Also shown is the basal tubulin self-polymerization in the absence of any additional protein (no protein). Values represent measurements performed in triplicate. Background absorbance was subtracted from each measurement. Values from a representative experiment are illustrated. (B) Average area under the curve values ± s.e.m. calculated from the tubulin polymerization curves shown in (A). The addition of CRMP2 increased polymerization, while the inclusion of 3 μM (R)- or (S)-LCM led to a significant reduction in CRMP2-mediated enhancement of tubulin polymerization AUC compared to DMSO (*p < 0.05; One-Way ANOVA, Bonferroni post-hoc analysis).