Figure 6.

(S)-LCM does not alter slow-inactivation of voltage-gated sodium channels. (A) Voltage protocol for slow inactivation. Currents were evoked by 5 s prepulses between −100 and +20 mV and then fast-inactivated channels were allowed to recover for 5 s at a hyperpolarized pulse to −100 mV. The fraction of channels available was determined by a 15 ms test pulse at −10 mV. (B) Representative peak Na⁺ currents, in response to a step to −10 mV following a prepulse at −100 mV (black trace) and −50 mV (gray trace) in neurons in the absence (left) or presence (right) of 300 μM (S)-LCM. (C) Summary of steady-state slow inactivation curves from cortical neurons in the absence or presence of 300 μM (S)-LCM applied acutely or overnight. (D) For comparison, the fraction of current available following a −50 mV prepulse is depicted. (S)-LCM did not alter sodium channel steady-state slow inactivation in cortical neurons at either time (p > 0.05) (Student's t-test) (n = 6).