Skip to main content
. 2014 Jul 24;8:196. doi: 10.3389/fncel.2014.00196

Figure 7.

Figure 7

The effect of (S)-LCM on neurite outgrowth phenocopies siRNA knockdown of CRMP2. (A) Representative inverted black and white representative image of a cortical neuron 48 h following EGFP-transfection. (B–F) Representative tracings of neurons transfected with EGFP ± control siRNA, CRMP2 siRNA, 200 μM (S)-LCM, or CRMP2 siRNA + (S)-LCM. (G) Total outgrowth of neurons transfected with EGFP, control siRNA, or CRMPsiRNA combined with 24 h (S)-LCM treatment (200 μM). CRMP2 siRNA and (S)-LCM reduced outgrowth to a similar level. Combination of CRMP2 and (S)-LCM did not produce a further reduction. (H–K) Comparison of the effects of CRMP2 siRNA and (S)-LCM on # of branches, # of processes, mean process length, and maximum process length. (*p < 0.05 vs. EGFP alone, One-Way ANOVA, Tukey's post-hoc analysis) (values represent mean ± s.e.m.) (n = 86–320 cells, 8 separate culture wells) (scale bar = 50 μm).