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. 2014 Jul 24;8:196. doi: 10.3389/fncel.2014.00196

Figure 8.

Figure 8

Targeting CRMP2 prevents activity-dependent increase in neurite outgrowth. (A) Timeline of experimental procedures. (B–E) Representative tracings of cortical neurons expressing EGFP and incubated for 96 h in vehicle, 25 mM KCl, 200 μM (S)-LCM, or 25 mM KCl + 200 μM (S)-LCM. (F) Total outgrowth of cortical neurons exposed to 25 mM KCl in the presence or absence of 200 μM (S)-LCM. (G) In naïve neurons, KCl exposure increased outgrowth by ~40% compared to vehicle. Co-application of (S)-LCM blunted the KCl-induced increase to ~10% (*p < 0.05 compared to control; Student's t-test) (n = 110–379 cells, across 8 separate culture wells) (scale bar = 50 μm).