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. 2014 Jul 24;8:196. doi: 10.3389/fncel.2014.00196

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Copyright © 2014 Wilson, Moutal, Melemedjian, Wang, Ju, François-Moutal, Khanna and Khanna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chronic KCl depolarization increases the association of CRMP2 and tubulin. (A) Timeline of experimental procedures. (B) Summary of CRMP2-binding to tubulin from cortical cell lysates as determined by ELISA. The values obtained from these experiments are arbitrary optical densities. Thus, we have normalized the values of treated groups to the control group, which was set to 100%. This allows us to compare values across experiments. KCl exposure increased CRMP2 binding by ~43.5% compared to control. As (S)-LCM was previously shown not to impact CRMP2/tubulin binding, its co-application did not alter the effect of KCl. (^*p < 0.05 compared to control; One-Way ANOVA, Tukey's post-hoc analysis) (n = 4).