Table 1.
Reagent | Concentration | Relative activity (%) |
---|---|---|
None | — | 100.0 |
DTT | 10 mM | 41.2 |
SDS | 10 mM | 11.3 |
Thiourea | 10 mM | 37.8 |
EDTA | 10 mM | 79.7 |
Tween-80 | 0.2% | 53.1 |
Triton X-100 | 0.2% | 19.0 |
H2O2 | 0.2% | 9.2 |
Mg2+ | 5 mM | 7.0 |
Zn2+ | 5 mM | 5.8 |
Ca2+ | 5 mM | 17.2 |
Cu2+ | 5 mM | 15.0 |
Ni2+ | 5 mM | 17.0 |
Mn2+ | 5 mM | 39.5 |
Fe2+ | 5 mM | 51.5 |
Fe3+ | 5 mM | 51.3 |
K+ | 5 mM | 26.8 |
Na+ | 0.5 M | 69.1 |
Na+ | 1.0 M | 37.0 |
Na+ | 1.5 M | 11.2 |