Figure 2.
BCAR3 and p130Cas colocalize to membrane ruffles in aggressive breast cancer cell lines. MCF-7, T47D, MDA-MB-231, and BT549 cells were plated onto fibronectin-coated coverslips in serum-containing medium, allowed to spread for 6 h, and processed for immunofluorescence. Cells were stained with antibodies recognizing p130Cas (A, D, G, and J) or BCAR3 (B, E, H, and K) and visualized by confocal microscopy as described in Materials and Methods. Merged images (C, F, I, and L). Arrowheads, sites of BCAR3 and p130Cas colocalization. Bar, 10 μm.
