Figure 5.

Depletion of BCAR3 from BT549 cells results in a loss of both BCAR3 and p130^Cas from membrane ruffles. BT549 cells were treated with vehicle (H₂O), control, or BCAR3-targeted siRNAs as described in Fig. 4. Forty-eight hours posttransfection, cells were replated onto fibronectin-coated coverslips in serum-containing medium, allowed to spread for 4 h, and processed for epifluorescence. BCAR3 and p130^Cas were visualized as described in Materials and Methods, maintaining equal exposure lengths for each antibody. Merged images (C, F, and I). Arrowheads, sites of BCAR3-p130^Cas colocalization at membrane ruffles. Arrows, sites of membrane ruffles devoid of BCAR3 and p130^Cas in cells treated with BCAR3-targeted siRNA (G–I).