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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Cancer Res. 2007 Jul 1;67(13):6174–6182. doi: 10.1158/0008-5472.CAN-06-3455

Figure 6.

Figure 6

Depletion of BCAR3 in BT549 cells results in an attenuated EGF response. A, BT549 cells were transfected with control or BCAR3-specific siRNAs and incubated for 48 h. Cells were then serum starved for 16 h and stimulated with 100 ng/mL of EGF for 0, 1, 5, or 30 min. p130Cas localization was visualized by epifluorescence with p130Cas antibodies (green) and filamentous actin was visualized by Texas red–phalloidin (red). Merged images are shown. B, control siRNA or BCAR3 siRNA-treated BT549 cells were seeded onto Boyden chambers 48 h posttransfection and allowed to migrate toward 100 ng/mL EGF for 6 h. *, P < 0.05 relative to control siRNA treatment of cells. C, BT549 cells were siRNA treated and stimulated with EGF as described in (A). p130Cas immune complexes were generated from 200 μg total cell lysates, separated by 8% SDS-PAGE, and immunoblotted with pTyr (top) or p130Cas (second panel) antibodies. Crk immune complexes were generated from 650 μg total cell lysates, separated by 8% SDS-PAGE, and immunoblotted with p130Cas (third panel) and Crk (bottom) antibodies.