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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Mol Cells. 2008 Dec 31;26(6):611–615.

Fig. 4.

Fig. 4

Global genomic hypomethylation at 180 base pair centromeric repeat region in DME:MET1 a/s transgenic plants. After McrBC digestion of genomic DNAs, the 180 base pair centromeric repeat region was amplified by PCR using primers shown in “Materials and Methods”. If DNA methylation is depleted, successful amplification is expected. Since 180 base pair centromeric repeat region is a repetitive sequence, ladder shape band was detected in late-flowering transgenic lines. NF, normal flowering transgenic line; LF, late flowering transgenic line.