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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Structure. 2012 Sep 13;20(11):1872–1880. doi: 10.1016/j.str.2012.08.018

Figure 2. POT1 Binds Overhangs Directionally from 3′ to 5′.

Figure 2

(A) Schematic of the G4 DNA with various dye positions.

(B and C) Schematic diagram representing the dye positions with respect to the individual OB fold binding sites. CT indicates C-terminal domain of POT1. The positions 24, 18, and 6 nt represent the distances between Cy3 and Cy5. The faint ovals indicate the OB unit bindings that should not induce FRET decrease. The step number shown in the single molecule traces (C) expected from individual OB fold binding is written above each oval. Single molecule traces obtained from the three DNA constructs (C). The dt indicates the time interval between POT1 addition and the first step of FRET decrease.

(D) The average dt from each DNA construct. The longest dt for the 6 nt DNA construct suggests POT1 binding initiates from the 3′ to 5′ direction. Error bars indicate the SEM.

(E) Schematic of a plausible POT1 binding mode.

See also Figure S2.