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. Author manuscript; available in PMC: 2015 Jul 14.
Published in final edited form as: Dev Cell. 2014 Jul 14;30(1):71–85. doi: 10.1016/j.devcel.2014.06.001

Figure 6. Neuronal autophagosomes do not form at ER exit sites.

Figure 6

(A) Still images and corresponding kymographs of GFP-Sec16L in primary DRG neurons show a decreasing gradient of ERES from the cell soma to the distal tip of the axon. ERES are enriched in the cell soma and proximal axon, and depleted from the distal axon. Kymographs show bidirectional/stationary motility of ERES along the axon and clearly display the decreasing density of ERES from cell soma to tip. Horizontal bar, 1 μm. Vertical bar, 1 min. (B) Time series of mCherry-Atg13 and GFP-Sec16L in the distal axon. Red arrowheads denote biogenesis events that form at sites distinct from or absent of ERES (green arrowheads). Scale bar, 1 μm. (C, D) Kymographs generated from the boxed regions in B. In C, the motility of Atg13 is not correlated with that of Sec16L, indicating that autophagosome biogenesis occurs independent of ERES. In D, autophagosomes are formed in the absence of ERES. Horizontal bar, 1 μm. Vertical bar, 1 min.