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. Author manuscript; available in PMC: 2014 Aug 28.
Published in final edited form as: Annu Rev Genet. 2013 Aug 28;47:121–137. doi: 10.1146/annurev-genet-111212-133522

Fig. 2. tRNA misacylation with Methionine.

Fig. 2

(A) tRNA misacylation with Met in mammalian cells is regulated by reactive oxygen species (ROS) which can be generated from both exogenous and endogenous sources. (B) HeLa cells exhibit a basal level of misacylation which increases further by ∼10-fold upon full induction. tRNA misacylation is detected by microarrays of total charged tRNA from cells pulse-labeled with 35S-Met (52). Array probes for tRNAMets are shown as black dots in the array layout. (C) In the codon tables, codons read by misacylated tRNAs under uninduced conditions are shown in red and under induced conditions are in red and pink. Met codon is in blue.