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. 2014 Aug;55(8):1702–1710. doi: 10.1194/jlr.M048918

Fig. 6.

Fig. 6.

Hsp90 regulates Pparγ activity. A: Western blot analysis of HEK293 cells cotransfected with CMV:FLAG-Pparγ and either FLAG-Hsp90 or empty vector. Densitometry is shown to the right. B: Cycloheximide treatment (50 μg/ml) of HEK293 cells transfected with CMV:FLAG-Pparγ and either CMV:FLAG-Hsp90 or vector. C: Immunoprecipitation of Pparγ from liver of chow-fed wild-type mice; (-) indicates isotype control. D: HEK293 cells transfected with both a CMV:FLAG-Pparγ and a PPRE luciferase construct and either vector or a FLAG:Hsp90 for 24 h followed by 16 h of treatment with vehicle or rosiglitazone (10 μM) before luciferase assay. Error bars represent SD, and * indicates P ≤ 0.05.