TABLE 1.

Retina ganglioside composition determined by two different lipid extraction methods

			Ganglioside Abundance (%)
Species	m/z	Molecular Ion	Upper Phase	Monophase	P
GD3
GD3(34:1)	720.8970	[M-2H]2−	24.09 ± 0.28	26.39 ± 0.90	a
GD3(36:2)	733.9048	[M-2H]2−	0.89 ± 0.17	0.33 ± 0.11	ns
GD3(36:1)	734.9126	[M-2H]2−	42.46 ± 0.29	43.01 ± 1.09	ns
GD3(38:1)	748.9283	[M-2H]2−	16.04 ± 0.48	16.86 ± 0.23	ns
GD3(40:1)	762.9439	[M-2H]2−	5.11 ± 0.22	4.66 ± 0.30	ns
GD3(42:2)	775.9517	[M-2H]2−	4.77 ± 0.31	4.75 ± 0.28	ns
GD3(42:1)	776.9596	[M-2H]2−	0.93 ± 0.57	—	ns
O-Ac-GD3(34:1)	741.9023	[M-2H]2−	0.77 ± 0.07	0.51 ± 0.10	ns
O-Ac-GD3(36:1)	755.9179	[M-2H]2−	3.23 ± 0.06	2.53 ± 0.10	ns
O-Ac-GD3(38:1)	769.9336	[M-2H]2−	0.52 ± 0.02	0.27 ± 0.01	ns
GD1
GD1(34:1)	903.4631	[M-2H]2−	0.33 ± 0.01	0.20 ± 0.34	ns
GD1(36:1)	917.4787	[M-2H]2−	55.52 ± 0.90	60.82 ± 2.53	a
GD1(38:1)	931.4944	[M-2H]2−	32.16 ± 1.06	32.25 ± 1.93	ns
GD1(40:1)	945.5100	[M-2H]2−	1.17 ± 0.12	1.29 ± 0.31	ns
O-Ac-GD1(36:1)	938.4840	[M-2H]2−	7.08 ± 0.22	3.73 ± 0.72	a
O-Ac-GD1(38:1)	952.4996	[M-2H]2−	3.74 ± 0.22	1.71 ± 0.21	ns
GT1
GT1(36:1)	1,063.0264	[M-2H]2−	39.27 ± 1.36	42.98 ± 1.31	a
GT1(38:4)	1,074.0186	[M-2H]2−	3.39 ± 0.66	5.02 ± 1.22	ns
GT1(38:1)	1,077.0421	[M-2H]2−	22.82 ± 0.99	21.73 ± 1.14	ns
O-Ac-GT1(36:1)	1,084.0317	[M-2H]2−	19.69 ± 0.37	19.02 ± 1.39	ns
O-Ac-GT1(38:4)	1,095.0239	[M-2H]2−	1.19 ± 0.56	1.06 ± 0.98	ns
O-Ac-GT1(38:1)	1,098.0473	[M-2H]2−	12.05 ± 0.32	8.81 ± 1.73	a
GQ1
GQ1(36:1)	1,208.5741	[M-2H]2−	34.30 ± 5.22	41.72 ± 21.86	ns
O-Ac-GQ1(36:1)	1,229.5794	[M-2H]2−	63.78 ± 4.65	54.62 ± 20.29	ns
GM3
GM3(34:1)	1,151.7058	[M-H]−	44.51 ± 5.40	60.49 ± 42.48	ns
GM3(36:1)	1,179.7371	[M-H]−	55.49 ± 5.40	39.5 ± 42.49	ns
		Total	26	25

Data are presented as mean ± standard deviation from n = 3 separate lipid extracts. Sample concentration: 1.0 mg/ml of original retina tissue extracted. O-Ac: O-acetylated headgroup; ns, not statistically significant.

^aP < 0.05.